Evaluation of Plasmid Mediated Colistin Resistance in Colistin Resistant Gram-negative Bacilli

Background: Infections caused by carbapenem-resistant gram-negative bacteria are treated with colonistin as a last resort. However, the increased resistance in recent years is significant for treating multiple drug-resistant infections. Objectives: This study aimed to determine genes mobile colistin (mcr)-1 and mcr-2 colistin-resistant isolates, understand mechanism, help treatment. Methods: Isolates were identified Vitek MS automated system. The antibiotic susceptibility was tested 2 Compact Enteric bacilli isolates stored at -20°C until molecular study. DNA extraction of performed boiling method. Then, polymerase chain reaction (PCR) optimization using specific primers, mcr-1 investigated multiplex PCR Results: About 170 enteric isolate mainly sent from internal medicine (44.7%) neurology (13.5%) services. According species identification, 37.6% Klebsiella pneumonia, 31.7% Serratia marcescens. Based on results, not detected isolates. Conclusions: Increased worrying discovery mcr require urgent precautions, even though did detect mcr-2. New studies investigating new needed mechanism identify resistant